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Total Bacterial Load via qPCR

Quantitative PCR (qPCR), also known as Real-Time PCR, is a method that measures the number of copies of a DNA region defined by a particular PCR primer(s). Using this method, we specifically amplify the 16S amplicon and quantify the total bacterial content in each sample to determine total bacterial load.

This technique can also be used as a means to determine the feasibility of your microbiome study by providing an exact measure of bacterial abundance in your samples. We highly recommend using this technique prior to sequencing samples suspected to have low biomass, to determine whether these samples will result in positive amplification.

Other Microbiome Sequencing Services

16S & 18S rRNA Sequencing

16S amplicon sequencing

Shotgun Metagenomic Sequencing

Parallel sequencing of DNA

Microbiome Analysis Service Inquiry